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alpaca immunization  (Sino Biological)


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    Sino Biological alpaca immunization
    Alpaca Immunization, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alpaca+immunization/pm41986718-764-11-5?v=Sino+Biological
    Average 95 stars, based on 37 article reviews
    alpaca immunization - by Bioz Stars, 2026-08
    95/100 stars

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    A dominant VHH clone that binds SARS-CoV-2 spike, saRBD-1, was isolated from an alpaca immunized with RBD (A) Schematic illustration of the immunization and VHH library construction pipeline. Alpacas were immunized over an 8-week period after which PBMC mRNA was isolated and processed into a VHH gene library. This library was transformed into phage-competent bacteria to generate a bacteriophage library, which was panned against SARS-CoV-2 S to enrich for binding clones. Clones were characterized through ELISAs on RBD and preliminary neutralization of S-pseudotyped lentivirus. (B) Representative immunofluorescence staining showing saRBD-1 VHH specifically associates with SARS-CoV-2 infected VeroE6 cells. Cells were infected with SARS-CoV-2 virus for 24 h. Fixed cells were stained with either saRBD-1 or control VHH (VHH52) followed by anti-VHH secondary (green).SARS-CoV-2 infection indicated by anti-dsRNA which stains replication centers (red). (C) SARS-CoV-2 S-transfected cells are specifically bound by saRBD-1 at levels detectable by flow cytometry. 293T cells transfected with full-length SARS-CoV-2 S were stained with either VHH saRBD-1 (green) or VHH52 (red) control. See also and .

    Journal: iScience

    Article Title: A potent alpaca-derived nanobody that neutralizes SARS-CoV-2 variants

    doi: 10.1016/j.isci.2022.103960

    Figure Lengend Snippet: A dominant VHH clone that binds SARS-CoV-2 spike, saRBD-1, was isolated from an alpaca immunized with RBD (A) Schematic illustration of the immunization and VHH library construction pipeline. Alpacas were immunized over an 8-week period after which PBMC mRNA was isolated and processed into a VHH gene library. This library was transformed into phage-competent bacteria to generate a bacteriophage library, which was panned against SARS-CoV-2 S to enrich for binding clones. Clones were characterized through ELISAs on RBD and preliminary neutralization of S-pseudotyped lentivirus. (B) Representative immunofluorescence staining showing saRBD-1 VHH specifically associates with SARS-CoV-2 infected VeroE6 cells. Cells were infected with SARS-CoV-2 virus for 24 h. Fixed cells were stained with either saRBD-1 or control VHH (VHH52) followed by anti-VHH secondary (green).SARS-CoV-2 infection indicated by anti-dsRNA which stains replication centers (red). (C) SARS-CoV-2 S-transfected cells are specifically bound by saRBD-1 at levels detectable by flow cytometry. 293T cells transfected with full-length SARS-CoV-2 S were stained with either VHH saRBD-1 (green) or VHH52 (red) control. See also and .

    Article Snippet: Spike immunized whole alpaca blood , Capralogics Inc. , N/A.

    Techniques: Isolation, Transformation Assay, Bacteria, Binding Assay, Clone Assay, Neutralization, Immunofluorescence, Staining, Infection, Virus, Control, Transfection, Flow Cytometry

    Journal: iScience

    Article Title: A potent alpaca-derived nanobody that neutralizes SARS-CoV-2 variants

    doi: 10.1016/j.isci.2022.103960

    Figure Lengend Snippet:

    Article Snippet: Spike immunized whole alpaca blood , Capralogics Inc. , N/A.

    Techniques: Recombinant, Virus, Electroporation, Purification, Labeling, Plasmid Preparation, Amplification, Software, Microscopy